TIBBİ AMAÇLAR İÇİN KULLANILAN BAZI BİTKİ TÜRLERİNİN ANTİMİKROBİYAL AKTİVİTELERİNİN ARAŞTIRILMASI

Abstract

Bu çalışmada; Ülkemizde doğal olarak yetişen ve çeşitli hastalıkların tedavisinde kullanılan Rhus coriaria L., Pistacia terebinthus L. sub. sp. palestina, Centaurea virgata Lav., Euphorbia macroclada Boiss., Ceterach officinarum DC., Echinophora tenuifolia L. sibthorpianal (Guss) Tutin, Equisetum romasissimum Desf., Umbilicus erectus DC. gibi sekiz bitki türünün, kloroform ekstraktları hazırlanarak disk difüzyon metoduna göre; Staphylococcus aureus COWAN 1, Bacillus megaterium DSM 32, Klebsiella pneumoniae FMC 66032, Escherichia coli ATCC 25922, Candida albicans FMC 17, Candida globrata ATCC 66032, Candida tropicalis ATCC 13803, Trichophyton sp., ve Epidermophyton sp. üzerinde antimikrobiyal etkileri test edildi. Ekstrelerin minimum inhibisyon konsantrasyonu mikrodilusyon yöntemine göre belirlendi. Çalışmalar sonucunda, kloroform ile hazırlanan Rhus coriaria L., Pistacia terebinthus L. sub. sp. palestina, ve Centaurea virgata Lav. bitki ekstraktlarının bakterilerin, mayaların ve dermofitlerin gelişmelerini değişen oranlarda engellediği, fakat Euphorbia macroclada Boiss., Ceterach officinarum DC., Echinophora tenuifolia L. sibthorpianal (Guss) Tutin, Equisetum romasisimum Desf., Umbilicus erectus DC. bitkilerinin; bazılarında etkili, bazılarında ise etkili olmadığı tespit edildi. Ayrıca aktif ekstrelerin MIC değerleri; 20-0.625mg/ml olarak değiştiği saptandı. Anahtar Kelimeler: Antimikrobiyal aktivite, Patojen mikroorganizmalar,Tıbbi bitkiler. I

In this study, eight species of plants extract, which grown as naturally in our country and used for treatment of various disease, were investigated. The extract of Rhus coriaria L., Pistacia terebinthus L. sub. sp. palestina, Centaurea virgata Lav., Euphorbia macroclada Boiss., Ceterach officinarum DC., Echinophora tenuifolia L. sibthorpianal (Guss) Tutin, Equisetum romasissimum Desf., Umbilicus erectus DC were prepared with chloroform, and antimicrobial activities of these extracts were examined on test microorganisms as follows: Staphylococcus aureus COWAN 1, Bacillus megaterium DSM 32, Klebsiella pneumoniae FMC 66032, Escherichia coli ATCC 25922 as bacteria species, Candida albicans FMC 17, Candida globrata ATCC 66032, Candida tropicalis ATCC 13803, Trichophyton sp., and Epidermophyton sp. by Disc Diffusion Methods. The MIC value of extract was determined as the method of micro dulitions. In the end of studies, we have found that the extracts of Rhus coriaria L., Pistacia terebinthus L. sub. sp. palestina, and Centaurea virgata Lav. prepared with chloroform, revealed antimicrobial activities against some bacteria, yeasts and dermatofit, but the extracts of Euphorbia macroclada Boiss., Ceterach officinarum DC., Echinophora tenuifolia L. sibthorpianal (Guss) Tutin, Equisetum romasisimum Desf., Umbilicus erectus DC prepared with chloroform, revealed antimicrobial activities against bacteria, yeasts and dermatofit, but also it had no antagonistic effect against some bacteria, yeasts and dermatofit used in this study. Also, MIC value of real extract was determined as 20-0.625 mg/ml. Key Words: Antimicrobial activity, Pathogen microorganizms, Medicinal plants.