Effects on the wound healing process using ozonated oils (Sesame, Nigella sativa, Hypericum perforatum) in rats

Abstract

Objective: In this study, the effects of three different ozonated oils (Sesame, Nigella sativa and Hypericumperforatum) on wound closure rate, healing process and possible complications were examinedmacroscopically and microscopically.Materials and Methods: Twenty-one adult Wistar albino female rats were used in the study. Subjects weredivided into three groups, early wound healing, (7 days), medium wound healing, (14 days) and late woundhealing (21 days). Four full-thickness skin wounds of equal size (10 mm in diameter) were formed on theback regions of all rats. This region was chosen for preventing self-inflicted injuries and reducing externalirritation. The wound was left open during the healing process. While the first wound (control) received notreatment in the second wound, ozonated Sesame oil, in the third wound ozonated Nigella sativa oil and inthe fourth wound ozonated Hypericum perforatum oil were used. No group obtained parenteral drugadministration. First, second and third main groups were euthanized on days 7, 14 and 21, respectively. Thewound healing was assessed macroscopically daily. Wound sizes of individual rats were measured with acaliper and digitally photographed every day from the day of injury. After euthanasia, all wound sites ofthe subjects were evaluated histopathologically.Results: There were no significant differences in wound healing between treatments in the first day 7. Onthey 14, it was found that the healing was better in the group applied Nigella sativa and Sesame oils (p?0.05).On day 21 wound healing was completed in all subjects with a resultant of better outcome in Nigella sativaoil treatment compared with others (p?0.01).Conclusion: In this study the best wound healing outcome was achieved with Nigella sativa oil and Sesameoil where three different minced oils were used.