Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors

Abstract

In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the diagnosability of superficial caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep with C. pseudotuberculosis were compared with healthy sheep, it was determined that some antioxidant molecule levels (such as GSH-Px (14.62%, p<0.001), GSH (23.81%, p<0.001), SOD (4.70%, p<0.001), CAT (22.23%, p<0.001). The level of toxic malondialdehyde (MDA) (18.62%, p<0.001). As a result, it was determined that oxidative stress factors in sheep with superficial caseous lymphadenitis (caused by C.pseudotuberculosis) showed statistically differed significantly compared to sheep in the control group. Therefore, even if there is no specific marker for the diagnosis of superficial and visceral forms of caseous lymphadenitis, the lev -els of oxidative stress factors in suspected flocks may suggest disease risk. In addition, this study is very important in order to form a basis for future studies on this subject. Although it has been determined that these biochemical markers are not pathognomonic for caseous lymphadenitis, it would be more accurate to associate them with not only oxidative stress factors but also many other factors in future studies.