Use of multiplex PCR for the diagnosis of Theileria annulata and Theileria buffeli

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dc.contributor.authorAltay, Kürşat
dc.contributor.authorAydin, Mehmet Fatih
dc.contributor.authorUluişik, U?ur
dc.contributor.authorAktaş, Münir
dc.contributor.authorDumanli, Nazir
dc.date.accessioned2026-08-12T16:13:08Z
dc.date.issued2008
dc.departmentFırat Üniversitesi
dc.description.abstractThe species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.
dc.identifier.endpage3
dc.identifier.issn1300-6320
dc.identifier.issue1
dc.identifier.pmid18351541
dc.identifier.scopus2-s2.0-61749090932
dc.identifier.scopusqualityQ3
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/11508/42850
dc.identifier.volume32
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isotr
dc.relation.ispartofTürkiye parazitolojii dergisi / Türkiye Parazitoloji Derne?i = Acta parasitologica Turcica / Turkish Society for Parasitology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20260511
dc.subjectAnimals; Antigens, Protozoan; Cattle; DNA, Protozoan; Electrophoresis, Agar Gel; Polymerase Chain Reaction; Protozoan Proteins; Theileria; Theileria annulata; Theileriasis; major piroplasm surface protein, Theileria; parasite antigen; protozoal DNA; protozoal protein; Tams1 antigen; unclassified drug; agar gel electrophoresis; animal; animal disease; article; cattle; genetics; isolation and purification; methodology; parasitology; pathogenicity; polymerase chain reaction; Theileria; Theileria annulata; theileriosis
dc.titleUse of multiplex PCR for the diagnosis of Theileria annulata and Theileria buffeli
dc.title.alternativeTheileria annulata ve Theileria buffeli'nin Teşhisinde Multiplex PCR'in Kullanilmasi.
dc.typeArticle

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