Evaluation of oxidative and antioxidant markers in the blood samples of patients with abdominal aorta pathology

Abstract

Objectives: This study aims to investigate the effects of oxidative damage on the development of the disease in patients scheduled for surgeryfor aortoiliac occlusive disease (AOD) and abdominal aortic aneurysm (AAA).Patients and methods: Between June 2015 and July 2016, a total of 24 patients (22 males, 2 females; mean age 63.2±10.4 years; range, 41to 76 years) who admitted to our clinic for surgery were included in the study. The study group consisted of all elective AOD and AAApatients. The control group (n=100) consisted of healthy volunteers including health care personnel, particularly physicians working in thehospital. We obtained blood samples before surgery and compared levels of malondialdehyde (MDA) as an indicator of oxidative damage,and the levels of certain antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) with theblood levels of healthy volunteers.Results: The comparison of the patient and healthy volunteer groups showed that blood MDA levels increased due to the oxidative damage(p=0.001). Regarding the antioxidant activity, we found that GSH levels increased in the patient group; however, the SOD activity remainedunchanged. The CAT activity was statistically significantly decreased in the patient group (p=0.001).Conclusion: The increased oxidative damage levels (MDA) in all patient groups compared to healthy individuals may indicate that oxidativestress has an important role in the development of the disease. In the light of these results, we conclude that the GSH activity is not sufficientto compensate the existing damage and the low CAT activity in all groups may show that it has a role in the development of the disease.