Investigation of Anaplasma Species with Veterinary and Public Health Significance in Sheep and Goats

Abstract

PurposeThis study was carried out to investigate Anaplasma important for veterinary and public health in sheep and goats in Ni & gbreve;de province in T & uuml;rkiye by using molecular methods.MethodsBlood samples were taken from randomly selected 690 animals (520 sheep and 170 goats), which were between 1 and 10 years old and from different study sites in Ni & gbreve;de by using the vacutainer tubes containing EDTA. After the genomic DNA extractions samples, the Anaplasma spp. 16S rRNA genes were amplified by PCR. Species-specific polymerase chain reaction (PCR) assays were performed on positive samples for the presence of A. bovis, A. capra, A. ovis, A. platys-like, and A. phagocytophilum. At the same time, the animals were tested for ixodid tick infestation and collected ticks were examined for identification under the stereo-microscope.ResultsThe results of PCR analysis show that the overall A. ovis prevalence was 63.3% (437/690) in small ruminants sampled. A total of 361 sheep (69.4%) and 76 goats (44.7%) were found to be infected with A. ovis, whereas no positivity was detected for A. bovis, A. capra, A. platys-like, and A. phagocytophilum. Anaplasma ovis positivity was observed at the highest percent in May (%74.6) while the lowest in June (%52.4). In total, 1361 ticks (579 female, 782 male) were collected from sheep and goats in Ni & gbreve;de. Ticks were identified as Rhipicephalus bursa (383, 28.1%), R. turanicus (607, 44.6%), Hyalomma marginatum (7, 0.5%), Hy. excavatum (247, 18.1%), Hy. anatolicum (23, 1.7%), Haemophsalis parva (21, 1.5%), Hae. punctata (7, 0.5%), Hae. sulcata (40, 2.9%) and Dermacentor marginatus (26, 1.9%).ConclusionThe present study reports a high prevalence of A. ovis 63.3% (437/690) in sheep and goats in Ni & gbreve;de province.