C60 Nanoparticle Decrease the Inflammatory and Oxidative Responses in 7,12-Dimethylbenz[a]Anthracene (DMBA) Induced Rats Eye Tissue

Abstract

Fullerene C-60 is known to have particularly strong antioxidant ability to protect against oxidative stress-induced dysfunctions and tissue damage. In this study, the preventive effects of C-60 were investigated in rats that caused eye tissue damage with 7,12-dimethylbenz[a]anthracene (DMBA). 60 Wistar albino female rats were used in the study. Groups: (i) Control Group: Group fed with standard diet; (ii) C-60 Group: Fullerene C-60 (1.7 mg/kg bw, oral gavage) given group; (iii) DMBA Group: The group given DMBA (45 mg/kg bw, oral gavage); (iv) C-60 + DMBA Group: Fulleren C-60 (1.7 mg/kg bw, oral gavage) and DMBA (45 mg/kg bw, oral gavage) group. Each group included 15 female rats and the study continued for 16 weeks. The protein levels were determined by western blotting and the biochemistry parameters were determined with spectrophotometric assays in rat eye tissue homogenates. Compared to the DMBA given group in the eye tissue, a decrease was observed in MDA levels and IL-1 & alpha; and COX-2 protein expression levels, and an increase in GSH level, CAT activity and caspase-3, HO-1, p53 and Beclin-1 expression levels in the C-60 given groups. Our results show that fullerene C-60 protects eye tissue from oxidative damage and reduces inflammation via trigging HO-1 expression.