An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids

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dc.contributor.authorAydin, Suleyman
dc.contributor.authorEmre, Elif
dc.contributor.authorUgur, Kader
dc.contributor.authorAydin, Mustafa Ata
dc.contributor.authorSahin, Ibrahim
dc.contributor.authorCinar, Vedat
dc.contributor.authorAkbulut, Taner
dc.date.accessioned2026-08-12T17:21:50Z
dc.date.issued2025
dc.departmentFırat Üniversitesi
dc.description.abstractThe enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field.
dc.identifier.doi10.1177/03000605251315913
dc.identifier.issn0300-0605
dc.identifier.issn1473-2300
dc.identifier.issue2
dc.identifier.orcid0000-0001-6162-3250
dc.identifier.pmid39922798
dc.identifier.scopus2-s2.0-85217404036
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1177/03000605251315913
dc.identifier.urihttps://hdl.handle.net/11508/54063
dc.identifier.volume53
dc.identifier.wosWOS:001415869000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltd
dc.relation.ispartofJournal of International Medical Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260511
dc.subjectBiological fluids, ELISA, antibody, antigen
dc.subjectenzyme-linked immunosorbent assay
dc.titleAn overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
dc.typeArticle

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