A comparison of faecal examination, commercial ELISA kit, and indirect-ELISA methods in the diagnosis of sheep fasciolosis

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dc.contributor.authorUtuk, Armagan Erdem
dc.contributor.authorSimsek, Sami
dc.contributor.authorKoroglu, Ergun
dc.date.accessioned2026-08-12T17:14:55Z
dc.date.issued2012
dc.departmentFırat Üniversitesi
dc.description.abstractThe aim of this study was to compare the diagnosis of sheep fasciolosis by faecal examination, commercial ELISA kit, and home-made indirect ELISA method. For this purpose, a total of 265 sheep faecal samples were examined with the sedimentation method and 185 sheep serum samples were examined both with a commercial ELISA kit and a home-made indirect ELISA method. The prevalence of Fasciola hepatica eggs was 4.9%. The prevalence of anti-F. hepatica antibodies was 16.2% using the commercial ELISA kit and 15.6% using the home-made indirect ELISA method. It is concluded that although the faecal examination is the gold standard in the diagnosis of sheep fasciolosis, serological tests should not be ruled out, and particularly the home-made indirect ELISA method may be preferred, since it is less expensive than commercial kits and has similar reliability rates. (C) 2012 Elsevier B.V. All rights reserved.
dc.description.sponsorshipCEVA-DIF
dc.description.sponsorshipThis work was partially supported financially by CEVA-DIF. The authors would like to thank to Dr. Georgios Theodoropoulos for the language edition.
dc.identifier.doi10.1016/j.smallrumres.2012.05.006
dc.identifier.endpage166
dc.identifier.issn0921-4488
dc.identifier.issue2.Mar
dc.identifier.orcid0000-0003-1841-4239
dc.identifier.orcid0000-0002-3567-326X
dc.identifier.scopus2-s2.0-84866501507
dc.identifier.scopusqualityQ2
dc.identifier.startpage164
dc.identifier.urihttps://doi.org/10.1016/j.smallrumres.2012.05.006
dc.identifier.urihttps://hdl.handle.net/11508/52019
dc.identifier.volume107
dc.identifier.wosWOS:000309441500016
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofSmall Ruminant Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260511
dc.subjectFasciola hepatica
dc.subjectCommercial ELISA kit
dc.subjectHome-made indirect ELISA
dc.subjectSheep
dc.titleA comparison of faecal examination, commercial ELISA kit, and indirect-ELISA methods in the diagnosis of sheep fasciolosis
dc.typeArticle

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