Response surface methodological approach for inclusion of perfluorocarbon in actinorhodin fermentation medium

Abstract

The combined effects of perfluorocarbon and glucose concentrations on actinothodin production by Streptomyces coelicolor A3(2) were studied in a 2-1 bioreactor using response surface methodology. A 2(2) full-factorial central composite design was employed for experimental design and analysis of the results. The optimum PFC and glucose concentrations were found to be 42.7% and 12.25 g/l, respectively. In these conditions, actinorhodin concentration of 60 mg/l with a biomass concentration of 1.9 g/l, a maximum volumetric oxygen uptake rate of 180 mgO(2)/l h and a glucose consumption rate of 0.110 g glucose/l h was attained. These results are in close agreement with the model predictions. (C) 2002 Elsevier Science Ltd. All rights reserved.