Detection of Chlamydophila abortus in ovine milk by Immunomagnetic separation-polymerase chain reaction

dc.contributor.authorÖngör, H
dc.contributor.authorÇetinkaya, B
dc.contributor.authorAçik, MN
dc.contributor.authorKarahan, M
dc.contributor.authorBulut, H
dc.date.accessioned2026-08-12T16:34:29Z
dc.date.issued2004
dc.departmentFırat Üniversitesi
dc.description.abstractThe present study was carried out to investigate the presence of Chlamydophila abortus, the causative agent of ovine enzootic abortion, in milk samples collected from sheep flocks with and without the history of abortion in Eastern Turkey by means of immunomagnetic separation (IMS) in conjunction with the polymerase chain reaction (PCR). A total number of 201 milk samples collected from 10 flocks with abortion and four flocks without abortion were tested. In the analysis of the milk samples by IMS-PCR, correct amplification was obtained with three (1.5%) samples originating from one flock with abortion. In the digestion of PCR positive products by AluI, restriction profiles observed in all three samples were determined to be the same as C. abortus S26/3.
dc.identifier.endpage45
dc.identifier.issn0931-1793
dc.identifier.issue1
dc.identifier.orcid0000-0002-1908-5898
dc.identifier.orcid0000-0002-5811-2588
dc.identifier.orcid0000-0003-4978-9578
dc.identifier.pmid14995977
dc.identifier.scopus2-s2.0-1342333350
dc.identifier.scopusqualityN/A
dc.identifier.startpage43
dc.identifier.urihttps://hdl.handle.net/11508/44457
dc.identifier.volume51
dc.identifier.wosWOS:000189102700008
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260511
dc.subjectChlamydia-Psittaci
dc.subjectGoats
dc.subjectPcr
dc.subjectStrain
dc.subjectElisa
dc.subjectSheep
dc.titleDetection of Chlamydophila abortus in ovine milk by Immunomagnetic separation-polymerase chain reaction
dc.typeArticle

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