Determination of Myricetin in Medicinal Plants by High-Performance Liquid Chromatography

Abstract

The concentrations of myricetin in medicinal plants such as Rosa canina L. (rosa hip), Terebinthina chica L. (terebinth), Urtica dioica L. (nettle), and Portuca oleracea L. (purslane) were determined by high-performance liquid chromatography-mass spectrometry (HPLC-MS). The aglycones of myricetin were extracted using methanol-ascorbic acid- hydrochloric acid, methanol-hydrochloric acid, and methanol, separated within 5min, and individually quantitated in the positive ionization mode using optimized conditions for HPLC-MS. Methanol-ascorbic acid-hydrochloric acid was the optimum extraction solvent. The myricetin concentration in the plants were between 3 and 58mg kg(-1), with a limit of quantification equal to 0.1mgL(-1.)