Ultrastructure of the Tongue of the German Mast Goose (Anser anser) by Scanning Electron Microscopy Before and After Plastination

Abstract

The aim of this study was to compare the micro anatomy of the tongue in German mast geese with other bird species by scanning electron microscopy (SEM) analysis. In addition, by applying the silicone plastination technique to the tongue, it is aimed to compare the SEM images before and after plastination. German mast geese used as study material were obtained from goose breeders. After the micro-anatomical features of the German goose tongue were determined, SEM (FEI-Quanta; FEG 250, USA) analyzes were made and their general structures were photographed. Then, silicone plastination processes were applied at room temperature. The SEM images of the fresh material and the plastinated material were compared by taking the SEM images again. As a result, it was determined that the features of the tongue were preserved macroscopically after the plastination process. The silicone plastination procedure of the German mast goose tissues took a total of 31 days. On the SEM plastination images, scattered dispersion was observed on the epithelium of the tongue surface, corresponding to the findings on the fresh material. The papillae on the SEM images were observed to be preserved as in the fresh material images.