A New Research on the Antioxidant Properties and Therapeutic Effects of Bee Pollen in Saccharomyces cerevisiae

Abstract

Bee pollen is a valuable product with apitherapeutic properties, highly valued by natural medicine for its medicinal and nutritional properties. Bee pollen, one of the basic elements of alternative therapy, has various biological activities such as antifungal, antiviral, antimicrobial, anti-inflammatory and antioxidant. In this study, four groups were created to investigate whether bee pollen has a protective role against copper chloride (CuCl2) damage in Saccharomyces cerevisiae. Study groups: (1) Control group: only yeast was cultivated; (2) CuCl2 group: given CuCl2 (30 mM); (3) Bee Pollen group: given bee pollen (10%); (4) Bee Pollen + CuCl2 group: given bee pollen (10%) + CuCl2 (30 mM). Saccharomyces cerevisiae cultures were grown at 30 degrees C for 1, 3, 5 and 24 hours. Cell growth, lipid peroxidation, MDA (malondialdehyde) analyzes, GSH (glutathione) levels and CAT (catalase) activities were determined by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and Lowry protein method. In the results of working; when compared to the CuCl2 group, cell development (1, 3, 5 and 24 hours), total protein synthesis and GSH level (24 hours) increased in bee pollen groups, while MDA level ( 24 hours) decreased. It has been determined that bee pollen has a role of promoting cell growth and total protein synthesis by reducing oxidative stress in Saccharomyces cerevisiae culture thanks to its antioxidant properties. Moreover, these natural products have been found to have a strong therapeutic effect in the treatment of many diseases.