Determination of immunogenic proteins of mumps virus by immunoblot assay

Abstract

The aim of this study was to determine the viral proteins of mumps virus infected VERO cells by using immunoblot technique performed after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein profiles of infected and non-infected cell lysates have been compared and 2 additional protein lines which have 60 and 20 kDa molecular weights were detected in infected cell samples after electrophoresis and staining with Coomassie blue, whereas there were no major-differences in protein patterns between them. In immunoblot assays performed by using high titered mumps virus specific haemagglutination inhibition antibodies and lysates of infected and control cells, 4 different protein lines which have 32, 60, 72 and 80 kDa molecular weights have been detected, supposed corresponding with matrix, fusion-1, nucleocapsid and haemagglutinin-neuraminidase proteins respectively which were the immunogenic proteins of the virus.