BIOPROCESS PARAMETERS AND OXYGEN TRANSFER CHARACTERISTICS IN PECTIN LYASE PRODUCTION BY BACILLUS PUMILUS

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dc.contributor.authorTepe, Ozlem
dc.contributor.authorDursun, Arzu Y.
dc.date.accessioned2026-08-12T16:58:33Z
dc.date.issued2017
dc.departmentFırat Üniversitesi
dc.description.abstractThe present study aimed at optimization of culture condition for the production of pectin lyase from Bacillus pumilus by submerged fermentation and investigation of the oxygen transfer effects on the enzyme production. Effects of initial pH, carbon and nitrogen sources, aeration, salts and phosphate on microbial growth, pectin lyase activity and released total protein were studied. Optimum pectin and ammonium sulphate concentrations were determined as % 1 (w/v) and 0.05 % (w/v) at pH:8, T:30 degrees C, 150 rpm, respectively. The maximum microorganism specific growth rate and pectin lyase activity were obtained as 0.0381 (h(-1)), 14.05 U/mL, respectively. The oxygen transfer coefficient (k(L)a) and oxygen uptake rate were also determined in a bioreactor. The results showed that, increases in the air feed rate increased the k(L)a values, B. pumilus produces mainly alkaline pectin lyase and the optimal pH and temperature for the activity were reported as 10 and 40 degrees C, respectively.
dc.description.sponsorshipFUBAP (The research Foundation of Firat University) [FUBAP 1674]
dc.description.sponsorshipThis paper includes a part of Pd.D thesis data of Ozlem TEPE. The authors wish to thank FUBAP (The research Foundation of Firat University), for the financial support of this study (Project No: FUBAP 1674).
dc.identifier.endpage5091
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue8
dc.identifier.orcid0000-0001-8336-3699
dc.identifier.startpage5082
dc.identifier.urihttps://hdl.handle.net/11508/46922
dc.identifier.volume26
dc.identifier.wosWOS:000409399600022
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260511
dc.subjectPectin lyase
dc.subjectBacillus pumilus. enzymes
dc.subjectoxygen transfer
dc.subjectvolumetric oxygen mass transfer coefficient
dc.titleBIOPROCESS PARAMETERS AND OXYGEN TRANSFER CHARACTERISTICS IN PECTIN LYASE PRODUCTION BY BACILLUS PUMILUS
dc.typeArticle

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